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Sec hplc column resistant to sodium hydroxide
Sec hplc column resistant to sodium hydroxide







sec hplc column resistant to sodium hydroxide

Azide solution, 65% Acetonitrile,) mentioned in COA of respective manufacturer for 15-20 minutes.

sec hplc column resistant to sodium hydroxide

  • Select shipping solvent, from COA of respective manufacturer, If not mentioned in COA, store the column based on column properties like Nitrile /ODS/Amine group etc.
  • Set column on column compartment of HPLC.
  • Pre-Wash (mobile phase run/ Prior to analysis) procedure
  • Do not over make tighter the column end fittings.
  • Keep column with proper end fitting during storage.
  • Do not change flow rate at increment greater than 0.2 ml/min.
  • Purge solvent line to make them free from air bubbles.
  • Freshly prepared, filtered and degassed solvent or solution should be used.
  • Use HPLC grade solvents for column washing.
  • To confirm the overall implementation of the SOP.
  • Take initiative to approval of the SOP.
  • To assemble training on the SOP to all concerned personnel and to confirm implementation of the SOP after training.
  • To ensure that the SOP reflects the required working practices.
  • To confirm that this procedure is kept up to date.
  • To keep record of HPLC column washing time properly.
  • To accomplish the HPLC column washing properly in accordance with this SOP.
  • To confirm that the instructions of this procedure are correctly followed.
  • sec hplc column resistant to sodium hydroxide

    The roles and responsibility is as follows Senior Executive/Executive, Quality Control

  • HPLC: High performance liquid chromatography.
  • This procedure is applicable for all HPLC columns used within quality control laboratory of XX Pharmaceuticals Limited. The tenacity of this SOP is to define the procedure for washing of HPLC column to avoid any intrusion on repeated usage & to escalation column lifespan.









    Sec hplc column resistant to sodium hydroxide